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Exiqon是RNA研究的灵活解决方案的*供应商，现在已成为QIAGEN的一部分。

Exiqon现在是QIAGEN公司

Exiqon和QIAGEN联手为您提供丰富的产品和服务，以两家公司的专业知识和产品协同效应为基础。Exiqon的产品和服务被世界各地的学术界，生物技术和制药公司的研究人员用于突破性地发现基因活动与疾病发展之间的相关性。利用我们专有的LNA™技术，我们的产品系列可为分析microRNA，mRNA和长非编码RNA提供的特异性和灵敏度。 Exiqon的产品和服务涵盖从样品制备到表达分析（测序，阵列和qPCR），体外和体内检测和功能分析的整个工作流程。 Exiqon Services提供RNA下一代测序，临床样品中microRNA分析和生物标志物发现方面的专业知识。

exiqon部分产品列表：

Cat No./ID: 76603

miRCURY Exosome Serum/Plasma Kit

Cat No./ID: 76743

miRCURY Exosome Cell/Urine/CSF Kit

miRCURY Exosome Kits产品介绍：

PerformanceEfficient exosome isolation from serum and plasma

The miRCURY Exosome Serum/Plasma Kit is optimized for isolation of exosomes from serum and plasma samples. This kit includes thrombin for efficient isolation of exosomes from plasma. To evaluate the efficiency of exosome isolation, we quantified the fraction of exosomes found in the pellet versus the supernatant, which is normally discarded (see figure Exosome recovery from serum). We observed a very high enrichment rate of extracellular vesicles. 

Efficient exosome isolation from cell-conditioned media, urine and CSF

The miRCURY Exosome Cell/Urine/CSF Kit is optimized for isolation of exosomes from cell-conditioned media, urine and CSF samples. In studies comparing the fraction of exosomes in the pellet versus supernatant for these sample types, we observed a very high exosome enrichment rate (see figures Exosome recovery from cell-conditioned media and Exosome recovery from urine). 

Typically, the amount of miRNA present in urine and CSF samples is very limited in healthy individuals. Therefore, we highly recommend performing an exosome isolation of these biofluids prior to RNA isolation and PCR profiling. This allows you to use the exosome isolation kit as a way of concentrating the sample, while keeping the amount of potential PCR inhibitors to a minimum (see figure Detect more miRNAs in biofluids using exosome isolation kits). 

Principle

The miRCURY Exosome Kits utilize precipitation to enrich for exosomes and other extracellular vesicles. They are optimized to provide efficient isolation of exosomes from various biofluid samples, such as cell-conditioned media, urine, CSF, serum or plasma. The protocols are easy to use and take just two hours. 

Although they are not species specific, the kit protocols are optimized for wong with human biofluids. Refer to the miRCURY Exosome Kits Handbook for recommended starting material for different sample types. 

Why study exosomes?

Exosomes are cell-derived, membranous particles ranging in size from 20–120 nm, approximately the same size as viruses but considerably smaller than microvesicles (see figure The structure of an exosome). Exosomes are excreted from cells into the surrounding media and can be found in many, if not all, body fluids. Their proposed role as intercellular hormone-like messengers, together with their stability as carrier of proteins and RNA, make them ideal as biomarkers for a variety of diseases and biological processes (see figure The hypothesis of exosomal shuttling of miRNAs). 

Exosomes are secreted by most cell types and are formed by the fusion of multivesicular bodies with the plasma membrane. They are believed to be involved in a number of functions, including: 

·         Immune regulation (e.g., tumor-derived exosomes may help tumors evade the immune response)

·         Blood coagulation

·         Cell migration

·         Cell differentiation

·         Cell-to-cell communication

Microvesicles that are larger than exosomes (up to 1 µm) are typically formed by blebbing of the plasma membrane, whereas exosomes are released via exocytosis from multivesicular bodies of the endosome. 

Procedure

Samples are centrifuged to remove cells and debris, as well as cryoprecipitates for previously frozen samples. Precipitation buffer is added, and the samples are incubated for 1 hout at room temperature. Exosomes are pelleted during a 30 minute centrifugation step. After resuspension, the exosomes are ready for downstream analysis.

Applications

The miRCURY Exosome Kits are mainly developed for miRNA/RNA profiling applications.

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