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Designation

Code

Antiserum against Cyclic AMP ( rabbit )

4A-001

lyophilized RIA-Titer:

1 : 75 000

Crossreactivity:

< 0.001 %

ATPcGMP

0.00%

Antiserum against Cyclic GMP ( rabbit )

4G-101

lyophilized RIA-Titer:

1 : 50 000

Crossreactivity: GTP

< 0.001 %

cAMP

< 0.001 %

Designation

Code

Endomorphin-1 ( Tyr-Pro-Trp-Phe-NH2 )
MW: 610.7 purity > 97 % (HPLC)

P-600

Endomorphin-2 ( Tyr-Pro-Phe-Phe-NH2 )
MW: 571.7 purity > 97 % (HPLC)

P-610

Subunit

Peptide

Binding Site

Specifity

Titer (blot)

Code

alpha
common

GAGESGK
STIVKQMK

GTP-binding core

alpha-o,
alpha-i1-3
alpha-1
>>alpha-s

3.51389

3A-100

alpha-o

NLKEDGIS
AAKDVK

Aminoacids
22-35 of alpha-o

alpha-o1
alpha-o2

1: 15 000

3A-110

alpha-o 1

SKNRSPN

Aminoacids 310-
316 of
alpha-o 1

alpha-o 1

1.43056

3A-120

alpha-i
1/2

KNNLKDCGLF

C-Terminus

alpha-i1, alpha
i2, alpha-t
>>alpha-i3

5.59722

3A-130

alpha-i 2

EEQGML
PEDLS

Aminoacids 115-
125 of
alpha-i2

alpha-i2

1.43056

3A-140

alpha-i 3

IDFGEAARAD

Aminoacids
93-102

alpha-i3

1.43056

3A-145

alpha-s

RMHLRQ
YELL

C-Terminus

alpha-s short
alpha-s long

1 : 5 000

3A-155

alpha-s

RMHLRQ
YELL

C-Terminus

alpha-s short
alpha-s long

1 : 75 000

3A-150

alpha-s

TPEPGED
PRVTR

3rd Loop

alpha-s short
alpha-s long

1.43056

3A-160

alpha-p
also termed
alpha-z or
alpha-x

QNNLKY
IGLC

C-Terminus

alpha-p/z/x

2.81944

3A-170

alpha
q=11

QLNLKE
YNLV

C-Terminus

alpha-q
alpha-11

2.81944

3A-180

alpha-12

QENLKD
IMLQ

C-Terminus

alpha-12

0.73611

3A-185

alpha-13

HDNLKQ
LMLQ

C-Terminus

alpha-13

0.73611

3A-190

alpha-14

QLNLRE
FNLV

C-Terminus

alpha-14

0.73611

3A-195

beta

EGNVRVSR
ELAGHTGY

Aminoacids
130-145 of beta-1

beta-35
beta-36

2.81944

3B-200

beta-2

CGDSTLTQ
ITAGLD

Aminoacids 25-38
of beta-2

beta-35

2.81944

3B-220

gamma-1

CEEFRD
YVEER

Aminoacids 8-18
of gamma-1

gamma-1
(Transducin

2.81944

3G-100

Subunit

Designation

Code

alpha-o
alpha-i
alpha-t

Cys-GAGESGKSTIVKQMK, GTP-binding core, acomm
Cys-Gly-Ala-Gly-Glu-Ser-Gly-Lys-Ser-Thr-Ile-Val-Lys-Gln-Met-Lys
> 98 % pure by HPLC
Aminoacids 40-54 of alpha-o/alpha-i/alpha-t
MW 1623.8

5-500

alpha-o

Cys-NLKEDGISAAKDVK, Aminoacids 22-35 of alpha-o,
Cys-Asn-Leu-Lys-Glu-Asp-Gly-Ile-Ser-Ala-Ala-Lys-Asp-Val-Lys
> 98 % pure by HPLC
MW 1590.7

5-510

alpha-i

LKDCGLF, C-Terminus of alpha-i1 or alpha-i2
Leu-Lys-Asp-Cys-Gly-Leu-Phe
< 80 % pure by HPLC
MW 794.9

5-550

EEQGMLPEDLS, Aminoacids 115-125 of alpha-i2
Glu-Glu-Gln-Gly-Met-Leu-Pro-Glu-Asp-Leu-Ser
< 80 % pure by HPLC
MW 1247.3

5-560

alpha-s

Cys-KQLQKDKQVYRATHR, Aminoacids 28-42 of alpha-s
Cys-Lys-Gln-Leu-Gln-Lys-Asp-Lys-Gln-Val-Tyr-Arg-Ala-Thr-His-Arg
> 98 % pure by HPLC
MW 2002.2

5-570

Cys-TPEPGEDPRVTR-amide, Aminoacids 325-336 of alpha-s
Cys-Thr-Pro-Glu-Pro-Gly-Glu-Asp-Pro-Arg-Val-Thr-Arg-amide (3rd loop)
< 80 % pure by HPLC
MW 1456.5

5-580

RMHLRQYELL, Aminoacids 384-394 of alpha-s
Arg-Met-His-Leu-Arg-Gln-Tyr-Glu-Leu-Leu
< 80% pure by HPLC
MW 1358.6

5-590

alpha-p

QNNLKYIGLC, Aminoacids 346-355 of alpha-p
Gln-Asn-Asn-Leu-Lys-Tyr-Ile-Gly-Leu-Cys
< 80 % by HPLC
MW 1165.3

5-600

alpha-q=11

CQLNLKEYNLV, Aminoacids 350-359 of alpha-q
Cys-Gln-Leu-Asn-Leu-Lys-Glu-Tyr-Asn-Leu-Val
85 % pure by HPLC
MW 1318.5

5-630

beta

Cys-EGNVRVSRELAGHTGY, Aminoacids 130-145 of beta-1
Cys-Glu-Gly-Asn-Val-Arg-Val-Ser-Arg-Glu-Leu-Ala-Gly-His-Thr-Gly-Tyr
> 98 % pure by HPLC
MW 1848

5-700

gamma-3

MKGETPVNSTI-Cys-amide, Aminoacids 1-12 of gamma-3
Met-Lys-Gly-Glu-Thr-Pro-Val-Asn-Ser-Thrr-Ile-Cys
> 70 % pure by HPLC
MW 1245

5-800