The A-Plus™ Poly(A) Polymerase Tailing Kit(Poly(A)加尾试剂盒)uses ATP as a substrate for template-independent addition of adenosine monophosphates to the 3'-hydroxyl termini of RNA.

Product Name: Poly(A)加尾试剂盒

Introduction:

The A-Plus™ Poly(A) Polymerase Tailing Kit uses ATP as a substrate for template-independent addition  of adenosine monophosphates to the 3'-hydroxyl termini of RNA. The standard protocol produces a  poly(A)-tail length of ~150 b on 40-60 μg of RNA. Polyadenylation increases the stability of RNA in  eukaryotic cells and enhances its ability to be translated after transfection or microinjection.1-3 A Poly(A)  tail is useful to provide a priming site for first-strand cDNA synthesis in certain applications, and can be  used to end-label4 or quantify5 mRNA.